产品货号：
GS4895
中文名称：
重组人UBE2R1(带His标签)
英文名称：
Recombinant Human Ubiquitin Conjugating Enzyme E2 R1, His
产品规格：
10μg
发货周期：
1～3天
产品价格：
询价
General Description
Ubiquitin-conjugating enzyme E2 R1 belongs to the ubiquitin-conjugating enzyme family and is encoded by the UBE2R1 gene in humans.The ubiquitin-conjugating enzymes,also known as E2 enzymes and more rarely as ubiquitin-carrier enzymes,take part in the second step in the ubiquitination reaction.In this reaction,E1 activates the ubiquitin by covalently attaching the molecule to its active site cysteine residue.The activated ubiquitin is then transferred to an E2 cysteine and then the E2 molecule binds E3 via a structurally conserved binding region.
UBE2R1 is important in the control of cell cycle and DNA replication.In vitro it catalyzes 'Lys-48'-linked polyubiquitination.Additionally,the protein cooperates with the UBCH5C and the SCF ligase complex for the polyubiquitination of NFKBIA leading to its subsequent proteasomal degradation.
Storage
This lyophilized preparation is stable at 2～8℃,but should be kept at -20℃ for long term storage,preferably desiccated.Upon reconstitution,the preparation is stable for up to one week at 2～8℃.For maximal stability,apportion the reconstituted preparation into working aliquots and store at -20℃ to -70℃.Avoid repeated freeze/thaw cycles.
Usage
This material is offered by bjbalb Biotech for research,laboratory or further evaluation purposes.NOT FOR HUMAN USE.
Molecular Weight
Approximately 26.7kDa,a single non-glycosylated polypeptide chain containing 236 amino acids.
Purity
>95% by SDS-PAGE and HPLC analyses.
Source
Escherichia coli.
Biological Activity
Data is not available.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
Lyophilized from a 0.2μm filtered concentrated solution in PBS,1mM DTT,pH7.5.
Endotoxin
Less than 0.1 EU/μg of rHuUBE2R1 is determined by LAL method.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom.Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1～1.0mg/ml.Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃.Further dilutions should be made in appropriate buffered solutions.
AA Sequence
MHHHHHHAMG ILMARPLVPS SQKALLLELK
GLQEEPVEGF RVTLVDEGDL YNWEVAIFGP
PNTYYEGGYF KARLKFPIDY PYSPPAFRFL
TKMWHPNIYE TGDVCISILH PPVDDPQSGE
LPSERWNPTQ NVRTILLSVI SLLNEPNTFS
PANVDASVMY RKWKESKGKD REYTDIIRKQ
VLGTKVDAER DGVKVPTTLA EYCVKTKAPA
PDEGSDLFYD DYYEDGEVEE EA
相关搜索：重组人UBE2R1(带His标签)，UBE2R1, His-tagged, Human
Ubiquitin-conjugating enzyme E2 R1 belongs to the ubiquitin-conjugating enzyme family and is encoded by the UBE2R1 gene in humans.The ubiquitin-conjugating enzymes,also known as E2 enzymes and more rarely as ubiquitin-carrier enzymes,take part in the second step in the ubiquitination reaction.In this reaction,E1 activates the ubiquitin by covalently attaching the molecule to its active site cysteine residue.The activated ubiquitin is then transferred to an E2 cysteine and then the E2 molecule binds E3 via a structurally conserved binding region.
UBE2R1 is important in the control of cell cycle and DNA replication.In vitro it catalyzes 'Lys-48'-linked polyubiquitination.Additionally,the protein cooperates with the UBCH5C and the SCF ligase complex for the polyubiquitination of NFKBIA leading to its subsequent proteasomal degradation.
Storage
This lyophilized preparation is stable at 2～8℃,but should be kept at -20℃ for long term storage,preferably desiccated.Upon reconstitution,the preparation is stable for up to one week at 2～8℃.For maximal stability,apportion the reconstituted preparation into working aliquots and store at -20℃ to -70℃.Avoid repeated freeze/thaw cycles.
Usage
This material is offered by bjbalb Biotech for research,laboratory or further evaluation purposes.NOT FOR HUMAN USE.
Molecular Weight
Approximately 26.7kDa,a single non-glycosylated polypeptide chain containing 236 amino acids.
Purity
>95% by SDS-PAGE and HPLC analyses.
Source
Escherichia coli.
Biological Activity
Data is not available.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
Lyophilized from a 0.2μm filtered concentrated solution in PBS,1mM DTT,pH7.5.
Endotoxin
Less than 0.1 EU/μg of rHuUBE2R1 is determined by LAL method.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom.Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1～1.0mg/ml.Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃.Further dilutions should be made in appropriate buffered solutions.
AA Sequence
MHHHHHHAMG ILMARPLVPS SQKALLLELK
GLQEEPVEGF RVTLVDEGDL YNWEVAIFGP
PNTYYEGGYF KARLKFPIDY PYSPPAFRFL
TKMWHPNIYE TGDVCISILH PPVDDPQSGE
LPSERWNPTQ NVRTILLSVI SLLNEPNTFS
PANVDASVMY RKWKESKGKD REYTDIIRKQ
VLGTKVDAER DGVKVPTTLA EYCVKTKAPA
PDEGSDLFYD DYYEDGEVEE EA
相关搜索：重组人UBE2R1(带His标签)，UBE2R1, His-tagged, Human